rabbit nonimmune serum (Santa Cruz Biotechnology)
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Rabbit Nonimmune Serum, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+nonimmune+serum/horseradish+peroxidase+conjugated+secondary+antibody/pm37023133-228-19-25
Average 90 stars, based on 1 article reviews
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Immunoprecipitation:Article Title: Circadian regulation of hippocampal function is disrupted with corticosteroid treatment Article Snippet: For each IP, chromatin was diluted 1:10 in ChIP dilution buffer [167 mM NaCl, 16.7 mM Tris-HCl (pH 8.1), 1.1% Triton X-100, 1.2 mM EDTA, 0.01% SDS] supplemented with complete protease inhibitor (Sigma). .. Reactions were immunoprecipitated overnight at 4 °C with 2 μg anti-GR M-20X (Santa Cruz Biotechnology, US) or Article Title: Circadian regulation of hippocampal function is disrupted with corticosteroid treatment. Article Snippet: For each IP, chromatin was diluted 1:10 in ChIP dilution buffer [167 mM NaCl, 16.7 mM Tris- HCl (pH 8.1), 1.1% Triton X- 100, 1.2 mM EDTA, 0.01% SDS] supplemented with complete protease inhibitor (Sigma). .. Reactions were immunoprecipitated overnight at 4 °C with 2 μg anti- GR M- 20X (Santa Cruz Biotechnology, US) or Negative Control:Article Title: Circadian regulation of hippocampal function is disrupted with corticosteroid treatment Article Snippet: For each IP, chromatin was diluted 1:10 in ChIP dilution buffer [167 mM NaCl, 16.7 mM Tris-HCl (pH 8.1), 1.1% Triton X-100, 1.2 mM EDTA, 0.01% SDS] supplemented with complete protease inhibitor (Sigma). .. Reactions were immunoprecipitated overnight at 4 °C with 2 μg anti-GR M-20X (Santa Cruz Biotechnology, US) or Article Title: Circadian regulation of hippocampal function is disrupted with corticosteroid treatment. Article Snippet: For each IP, chromatin was diluted 1:10 in ChIP dilution buffer [167 mM NaCl, 16.7 mM Tris- HCl (pH 8.1), 1.1% Triton X- 100, 1.2 mM EDTA, 0.01% SDS] supplemented with complete protease inhibitor (Sigma). .. Reactions were immunoprecipitated overnight at 4 °C with 2 μg anti- GR M- 20X (Santa Cruz Biotechnology, US) or Control:Article Title: Expression of Fas and Fas-ligand and analysis of argyrophilic nucleolar organizer regions in squamous cell carcinoma: relationships with tumor stage and grade, and apoptosis. Article Snippet: The aim of this study was to investigate whether levels of Fas and Fas-ligand (Fas-L) expression in oral and oropharyngeal squamous cell carcinomas (SCCs) are associated with tumor stage and grade, and to assess whether parameters related to argyrophilic nucleolar organizer regions (AgNORs) correlate with apoptosis in SCC cells and adjacent normal epithelium.. Fifty-two specimens taken from the tumoral and neighboring normal tissues of 26 patients with oral and oropharyngeal SCC were analyzed for Fas/Fas-L expression, and 24 specimens from 12 patients for AgNOR parameters.. Seventeen (65%) of the tumors were Fas and/or Fas-L-positive (by immunohistochemistry). |
