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rabbit nonimmune serum  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology rabbit nonimmune serum
    Rabbit Nonimmune Serum, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+nonimmune+serum/horseradish+peroxidase+conjugated+secondary+antibody/pm37023133-228-19-25
    Average 90 stars, based on 1 article reviews
    rabbit nonimmune serum - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: Circadian regulation of hippocampal function is disrupted with corticosteroid treatment
    Article Snippet: For each IP, chromatin was diluted 1:10 in ChIP dilution buffer [167 mM NaCl, 16.7 mM Tris-HCl (pH 8.1), 1.1% Triton X-100, 1.2 mM EDTA, 0.01% SDS] supplemented with complete protease inhibitor (Sigma). .. Reactions were immunoprecipitated overnight at 4 °C with 2 μg anti-GR M-20X (Santa Cruz Biotechnology, US) or rabbit nonimmune serum (2μg sc-2027; Santa Cruz, USA) for the negative control. .. GR–DNA complexes were collected onto protein A–conjugated Dynabeads (Invitrogen, Paisley, UK) and washed to remove nonspecific binding.

    Article Title: Circadian regulation of hippocampal function is disrupted with corticosteroid treatment.
    Article Snippet: For each IP, chromatin was diluted 1:10 in ChIP dilution buffer [167 mM NaCl, 16.7 mM Tris- HCl (pH 8.1), 1.1% Triton X- 100, 1.2 mM EDTA, 0.01% SDS] supplemented with complete protease inhibitor (Sigma). .. Reactions were immunoprecipitated overnight at 4 °C with 2 μg anti- GR M- 20X (Santa Cruz Biotechnology, US) or rabbit nonimmune serum (2μg sc- 2027; Santa Cruz, USA) for the negative control. .. GR–DNA complexes were collected onto protein A–conjugated Dynabeads (Invitrogen, Paisley, UK) and washed to remove nonspecific binding.

    Negative Control:

    Article Title: Circadian regulation of hippocampal function is disrupted with corticosteroid treatment
    Article Snippet: For each IP, chromatin was diluted 1:10 in ChIP dilution buffer [167 mM NaCl, 16.7 mM Tris-HCl (pH 8.1), 1.1% Triton X-100, 1.2 mM EDTA, 0.01% SDS] supplemented with complete protease inhibitor (Sigma). .. Reactions were immunoprecipitated overnight at 4 °C with 2 μg anti-GR M-20X (Santa Cruz Biotechnology, US) or rabbit nonimmune serum (2μg sc-2027; Santa Cruz, USA) for the negative control. .. GR–DNA complexes were collected onto protein A–conjugated Dynabeads (Invitrogen, Paisley, UK) and washed to remove nonspecific binding.

    Article Title: Circadian regulation of hippocampal function is disrupted with corticosteroid treatment.
    Article Snippet: For each IP, chromatin was diluted 1:10 in ChIP dilution buffer [167 mM NaCl, 16.7 mM Tris- HCl (pH 8.1), 1.1% Triton X- 100, 1.2 mM EDTA, 0.01% SDS] supplemented with complete protease inhibitor (Sigma). .. Reactions were immunoprecipitated overnight at 4 °C with 2 μg anti- GR M- 20X (Santa Cruz Biotechnology, US) or rabbit nonimmune serum (2μg sc- 2027; Santa Cruz, USA) for the negative control. .. GR–DNA complexes were collected onto protein A–conjugated Dynabeads (Invitrogen, Paisley, UK) and washed to remove nonspecific binding.

    Control:

    Article Title: Expression of Fas and Fas-ligand and analysis of argyrophilic nucleolar organizer regions in squamous cell carcinoma: relationships with tumor stage and grade, and apoptosis.
    Article Snippet: The aim of this study was to investigate whether levels of Fas and Fas-ligand (Fas-L) expression in oral and oropharyngeal squamous cell carcinomas (SCCs) are associated with tumor stage and grade, and to assess whether parameters related to argyrophilic nucleolar organizer regions (AgNORs) correlate with apoptosis in SCC cells and adjacent normal epithelium.. Fifty-two specimens taken from the tumoral and neighboring normal tissues of 26 patients with oral and oropharyngeal SCC were analyzed for Fas/Fas-L expression, and 24 specimens from 12 patients for AgNOR parameters.. Seventeen (65%) of the tumors were Fas and/or Fas-L-positive (by immunohistochemistry).



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    Image Search Results


    LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit IgG and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.

    Journal: American Journal of Cancer Research

    Article Title: REST-repressed lncRNA LINC01801 induces neuroendocrine differentiation in prostate cancer via transcriptional activation of autophagy

    doi:

    Figure Lengend Snippet: LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit IgG and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.

    Article Snippet: Antibodies were used in ChIP assays as follows: anti-REST (Millipore, 17-641) and nonimmune rabbit serum IgG (Alpha Diagnostic International).

    Techniques: ChIP-sequencing, Binding Assay, Amplification, Quantitative RT-PCR